Journal: Analytical Chemistry
Article Title: Evolution and Impact of Nucleic Acid Amplification Test (NAAT) for Diagnosis of Coronavirus Disease
doi: 10.1021/acs.analchem.3c05225
Figure Lengend Snippet: Schematic representation of the SARS-CoV-2 genome, which includes a single-stranded RNA having various Open Reading Frames (ORF1a, ORF1b, ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8a, ORF8b, ORF9b), leading to the formation of 16 nonstructural protein (nsps1–nsps16), structural proteins like S (spike protein) having the S1 subunit, which includes NTD, RBD, SD1, and SD2 and the S2 subunit, which contains FL, HR1, HR2, and TM. Other structural proteins are E (Envelop protein), M (Membrane protein), N (Nucleocapsid protein). PL-pro (papain-like protease), and 3CL-pro (cysteine proteases). [Created with BioRender.com .]
Article Snippet: Different LoDs have been observed for the SARS-CoV-2 MassArray Test (0.69–2.75 copies/mL), Agena SARS-CoV-2 Panel (2.5 copies/mL), and Ethos Laboratories SARS-CoV-2 MALDI-TOF Assay (1 TCID50/mL), with PPA and NPA values for the three tests ranging from 95% to 100%.
Techniques: Membrane